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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1983-10-8
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pubmed:abstractText |
Southern blot analysis suggests that multiple sequences homologous to a phenobarbital-inducible cytochrome P-450 cDNA are present in the rat and mouse genomes. A cDNA (pP-450b-5) to a major phenobarbital-inducible cytochrome P-450 mRNA species in the rat detected 6 polymorphic DNA fragments when hybridized to DNA from C57BL/6J and DBA/2J mice restricted with endonucleases EcoRI, BamHI, and PvuII. Using the BXD recombinant inbred strains, five of these polymorphisms were mapped to the Coh (coumarin hydroxylase) locus on chromosome 7 of the mouse. The Coh locus has previously been shown to code for a phenobarbital-inducible enzyme, believed to be a cytochrome P-450, which catalyzes the conversion of coumarin to 7-hydroxycoumarin (umbelliferone). The DNA polymorphisms appear to reflect changes in either cytochrome P-450 genes or pseudogenes that are very closely linked to the gene responsible for differential coumarin hydroxylase in mice or it may represent a change(s) in the Coh gene itself. The region of the Coh locus on chromosome 7 may be the site of a cluster of cytochrome P-450 genes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
258
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9585-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6309775-Animals,
pubmed-meshheading:6309775-Chromosome Mapping,
pubmed-meshheading:6309775-Cytochrome P-450 Enzyme System,
pubmed-meshheading:6309775-DNA,
pubmed-meshheading:6309775-DNA Restriction Enzymes,
pubmed-meshheading:6309775-Deoxyribonuclease EcoRI,
pubmed-meshheading:6309775-Deoxyribonuclease HindIII,
pubmed-meshheading:6309775-Enzyme Induction,
pubmed-meshheading:6309775-Isoenzymes,
pubmed-meshheading:6309775-Mice,
pubmed-meshheading:6309775-Mice, Inbred Strains,
pubmed-meshheading:6309775-Phenobarbital,
pubmed-meshheading:6309775-Polymorphism, Genetic,
pubmed-meshheading:6309775-Rabbits,
pubmed-meshheading:6309775-Rats,
pubmed-meshheading:6309775-Rats, Inbred Strains
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pubmed:year |
1983
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pubmed:articleTitle |
Genetic polymorphisms for a phenobarbital-inducible cytochrome P-450 map to the Coh locus in mice.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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