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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1983-9-23
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pubmed:abstractText |
Single Ca channel currents in tissue cultured clonal cells (GH3) isolated from a rat anterior pituitary tumour were studied with a patch voltage-clamp electrode filled with 100 mM-BaCl2 solution. The open time histogram was reasonably well expressed by a single exponential function with a time constant, which was about 1 msec and almost voltage-independent in the range of membrane potential studied (-20 to +40 mV). The closed time histogram was expressed by a sum of two exponential functions, one with a relatively voltage-independent time constant of about 1 msec and the other with a substantially larger voltage-dependent time constant. The latency (the time from the onset of the voltage-clamp pulse to the first channel opening) histogram could be expressed as the difference of two exponential functions with time constants which appeared to be voltage-dependent. The tentative conclusion is that the channel enters the activated state with voltage-dependent and relatively slow kinetics, and then flickers between open and closed states with substantially faster and relatively voltage-independent kinetics. The amplitude of the single channel current was similar to that estimated from noise analysis of the whole cell current (about -0.7 pA at 0 mV in 100 mM-BaCl2 solution); the apparent slope conductance was about 7-8 pS.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-1177128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-381636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-4951281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6111797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6246440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6262657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6262761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6272286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6272298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6281357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6289246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6296367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6296371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-6296372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6308233-7069629
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
336
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
649-61
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1983
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pubmed:articleTitle |
Studies of single calcium channel currents in rat clonal pituitary cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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