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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1983-9-23
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pubmed:abstractText |
A sample of colonies from the Clarke-Carbon ColE1-Escherichia coli DNA plasmid gene bank was screened by conjugation for complementation of the lipoamide dehydrogenase lesion of a deletion strain lacking all components of the pyruvate dehydrogenase complex, delta (aroP aceE aceF lpd). Two ColE1-lpd+ hybrid plasmids were identified: pGS2 (ColE1-ace lpd+; 24 kb) and pGS5 (ColE1-lpd+; 14 kb). Enzymological studies confirmed that pGS2 expressed all the activities of the pyruvate dehydrogenase complex, whereas pGS5 expressed the lipoamide dehydrogenase and acetyltransferase activities (the latter from a ColE1 promoter). These and other plasmids were used to construct a 47-site (15 enzymes) restriction map for a 24.2 kb segment of bacterial DNA in the nadC-lpd region. A further 13 sites (six enzymes) were defined in a 5.4 kb sub-segment containing the lpd gene. lambda phage derivatives containing specific fragments were constructed and used in transduction studies which located the ace and lpd genes in a 7.78 kb sub-segment flanked by AccI and NruI sites.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1287
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
671-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6308128-Bacteriophage lambda,
pubmed-meshheading:6308128-Chromosome Mapping,
pubmed-meshheading:6308128-Chromosomes, Bacterial,
pubmed-meshheading:6308128-Conjugation, Genetic,
pubmed-meshheading:6308128-DNA, Bacterial,
pubmed-meshheading:6308128-DNA Restriction Enzymes,
pubmed-meshheading:6308128-Escherichia coli,
pubmed-meshheading:6308128-Genes, Bacterial,
pubmed-meshheading:6308128-Genotype,
pubmed-meshheading:6308128-Hybridization, Genetic,
pubmed-meshheading:6308128-Plasmids,
pubmed-meshheading:6308128-Pyruvate Dehydrogenase Complex,
pubmed-meshheading:6308128-Transduction, Genetic
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pubmed:year |
1983
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pubmed:articleTitle |
Hybrid plasmids containing the pyruvate dehydrogenase complex genes and gene-DNA relationships in the 2 to 3 minute region of the Escherichia coli chromosome.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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