Linked Life Data

6307100

Source:http://linkedlifedata.com/resource/pubmed/id/6307100

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-8-11
pubmed:abstractText
An enzyme capable of degrading type V collagen was found in the sputum of patients with chronic obstructive pulmonary disease (COPD). This enzyme was found in a latent form and was activated by 4-aminophenylmercuric acetate (APMA). Molecular sieve chromatography on Ultrogel AcA-34 and affinity chromatography on type V collagen-Sepharose were successful in separating type V from type I collagenolytic activity. The enzyme became active after affinity chromatography and no longer required activation with APMA to manifest activity. It was found to be a metalloproteinase by virtue of its inhibition by ethylene-diaminetetraacetic acid and phenanthroline but not by phenylmethanesulfonyl fluoride or N-ethylmaleimide. Because degradation of matrix proteins is felt to be important in the pathogenesis of COPD, it is possible that the selective degradation of type V collagen by this enzyme may play a role in the development of COPD.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0003-0805
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
166-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Identification of a type V collagen-degrading enzyme from human sputum.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.