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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1983-8-11
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pubmed:abstractText |
The genome of Pf3, a filamentous single-stranded DNA bacteriophage of Pseudomonas aeruginosa (a gram-negative organism) was cloned into pBD214, a plasmid cloning vector of Bacillus subtilis (a gram-positive organism). Cloning in the gram-positive organism was done to avoid anticipated lethal effects. The entire Pf3 genome was inserted in each orientation at a unique Bc/I site within a thymidylate synthetase gene (from B. subtilis phage beta 22) on the plasmid. Additional clones were made by inserting EcoRI fragments of Pf3 DNA into a unique EcoRI site within this gene.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
221-3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6306273-Bacillus subtilis,
pubmed-meshheading:6306273-Bacteriophages,
pubmed-meshheading:6306273-DNA, Viral,
pubmed-meshheading:6306273-DNA Replication,
pubmed-meshheading:6306273-Electrophoresis, Agar Gel,
pubmed-meshheading:6306273-Genes, Bacterial,
pubmed-meshheading:6306273-Genetic Vectors,
pubmed-meshheading:6306273-Phenotype,
pubmed-meshheading:6306273-Plasmids,
pubmed-meshheading:6306273-Pseudomonas aeruginosa
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pubmed:year |
1983
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pubmed:articleTitle |
Cloning of Pf3, a filamentous bacteriophage of Pseudomonas aeruginosa, into the pBD214 vector of Bacillus subtilis.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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