| pubmed-article:6302990 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6302990 | lifeskim:mentions | umls-concept:C0026630 | lld:lifeskim |
| pubmed-article:6302990 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:6302990 | lifeskim:mentions | umls-concept:C0032583 | lld:lifeskim |
| pubmed-article:6302990 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:6302990 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:6302990 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6302990 | pubmed:dateCreated | 1983-6-10 | lld:pubmed |
| pubmed-article:6302990 | pubmed:abstractText | Two revertants of ts110 Moloney murine sarcoma virus (MuSV) with wild-type MuSV phenotype were examined for the presence of mos gene products, ts110 MuSV has a temperature-sensitive defect in a function required to maintain the transformed phenotype. The nonproducer 6m2 cell clone transformed by ts110 produces an 85,000-Da gag-mos protein (P85gag-mos) and a 58,000-Da gag protein (P58gag). A spontaneous revertant (clone 54-5A4) of the 6m2 cell clone produces a 100,000-Da protein (P100) recognized by antisera raised against murine leukemia virus p15, p12, and p30 but lacks determinants of p10, reverse transcriptase, and gp70. P100 was specifically recognized by antisera (anti-C3) prepared against a synthetic peptide representing the predicted C-terminal 12 amino acids of Moloney MuSV v-mos gene. Normal sera or anti-C3 blocked with excess synthetic peptide did not recognize P100. Thus, P100 is a product of the gag and mos genes. P100 was found to be phosphorylated. A second wild-type revertant (clone 204-3) was obtained by superinfection of ts110 nonproducer cells with Simian sarcoma associated virus (SSAV); it was also found to contain a phosphorylated P100gag-mos protein. The 204-3 cell clone also contained two gag polyproteins (Pr60gag and Pr55gag) of the size and antigenic properties of those found in SSAV-infected cells. These results provide two examples of P100 gag-mos proteins both derived from the P85gag-mos producing 6m2 cell clone. The P100 gag-mos polyproteins are made in amounts that are easily detected by radiolabeling experiments using [3H]leucine. The intracellular viral RNAs present in 6m2 cells and the two revertant clones were also examined. All three cell clones contained a 4.0 kb RNA hybridizing to v-mos sequences but only the 6m2 clone contained a 3.5 kb mos-containing RNA. Our findings indicate that the 3.5 kb RNA codes for P85gag-mos in cell-free translation experiments (Junghans et al., 1982, J. Mol. Biol. 161, 229). These findings as they relate to the mechanism that produces P100gag-mos instead of P85gag-mos are discussed. | lld:pubmed |
| pubmed-article:6302990 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6302990 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6302990 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6302990 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6302990 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6302990 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6302990 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6302990 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6302990 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6302990 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6302990 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:6302990 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:6302990 | pubmed:author | pubmed-author:HortJ FJF | lld:pubmed |
| pubmed-article:6302990 | pubmed:author | pubmed-author:ArlinghausR... | lld:pubmed |
| pubmed-article:6302990 | pubmed:author | pubmed-author:GallickG EGE | lld:pubmed |
| pubmed-article:6302990 | pubmed:author | pubmed-author:MurphyE CECJr | lld:pubmed |
| pubmed-article:6302990 | pubmed:author | pubmed-author:KloetzerW SWS | lld:pubmed |
| pubmed-article:6302990 | pubmed:author | pubmed-author:BlairD GDG | lld:pubmed |
| pubmed-article:6302990 | pubmed:author | pubmed-author:StankerL HLH | lld:pubmed |
| pubmed-article:6302990 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6302990 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:6302990 | pubmed:volume | 126 | lld:pubmed |
| pubmed-article:6302990 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6302990 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6302990 | pubmed:pagination | 336-47 | lld:pubmed |
| pubmed-article:6302990 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:6302990 | pubmed:meshHeading | pubmed-meshheading:6302990-... | lld:pubmed |
| pubmed-article:6302990 | pubmed:meshHeading | pubmed-meshheading:6302990-... | lld:pubmed |
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| pubmed-article:6302990 | pubmed:meshHeading | pubmed-meshheading:6302990-... | lld:pubmed |
| pubmed-article:6302990 | pubmed:meshHeading | pubmed-meshheading:6302990-... | lld:pubmed |
| pubmed-article:6302990 | pubmed:meshHeading | pubmed-meshheading:6302990-... | lld:pubmed |
| pubmed-article:6302990 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6302990 | pubmed:articleTitle | Gag-mos Polyproteins encoded by variants of the Moloney strain of mouse sarcoma virus. | lld:pubmed |
| pubmed-article:6302990 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6302990 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6302990 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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