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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1983-6-10
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pubmed:abstractText |
Two revertants of ts110 Moloney murine sarcoma virus (MuSV) with wild-type MuSV phenotype were examined for the presence of mos gene products, ts110 MuSV has a temperature-sensitive defect in a function required to maintain the transformed phenotype. The nonproducer 6m2 cell clone transformed by ts110 produces an 85,000-Da gag-mos protein (P85gag-mos) and a 58,000-Da gag protein (P58gag). A spontaneous revertant (clone 54-5A4) of the 6m2 cell clone produces a 100,000-Da protein (P100) recognized by antisera raised against murine leukemia virus p15, p12, and p30 but lacks determinants of p10, reverse transcriptase, and gp70. P100 was specifically recognized by antisera (anti-C3) prepared against a synthetic peptide representing the predicted C-terminal 12 amino acids of Moloney MuSV v-mos gene. Normal sera or anti-C3 blocked with excess synthetic peptide did not recognize P100. Thus, P100 is a product of the gag and mos genes. P100 was found to be phosphorylated. A second wild-type revertant (clone 204-3) was obtained by superinfection of ts110 nonproducer cells with Simian sarcoma associated virus (SSAV); it was also found to contain a phosphorylated P100gag-mos protein. The 204-3 cell clone also contained two gag polyproteins (Pr60gag and Pr55gag) of the size and antigenic properties of those found in SSAV-infected cells. These results provide two examples of P100 gag-mos proteins both derived from the P85gag-mos producing 6m2 cell clone. The P100 gag-mos polyproteins are made in amounts that are easily detected by radiolabeling experiments using [3H]leucine. The intracellular viral RNAs present in 6m2 cells and the two revertant clones were also examined. All three cell clones contained a 4.0 kb RNA hybridizing to v-mos sequences but only the 6m2 clone contained a 3.5 kb mos-containing RNA. Our findings indicate that the 3.5 kb RNA codes for P85gag-mos in cell-free translation experiments (Junghans et al., 1982, J. Mol. Biol. 161, 229). These findings as they relate to the mechanism that produces P100gag-mos instead of P85gag-mos are discussed.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0042-6822
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
126
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
336-47
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6302990-Animals,
pubmed-meshheading:6302990-Cell Line,
pubmed-meshheading:6302990-Cell Transformation, Neoplastic,
pubmed-meshheading:6302990-Cell Transformation, Viral,
pubmed-meshheading:6302990-Genes, Viral,
pubmed-meshheading:6302990-Mutation,
pubmed-meshheading:6302990-Oncogenes,
pubmed-meshheading:6302990-Phosphoproteins,
pubmed-meshheading:6302990-RNA, Viral,
pubmed-meshheading:6302990-Rats,
pubmed-meshheading:6302990-Sarcoma Viruses, Murine,
pubmed-meshheading:6302990-Temperature,
pubmed-meshheading:6302990-Viral Proteins
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pubmed:year |
1983
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pubmed:articleTitle |
Gag-mos Polyproteins encoded by variants of the Moloney strain of mouse sarcoma virus.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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