Linked Life Data

6300994

Source:http://linkedlifedata.com/resource/pubmed/id/6300994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-5-5
pubmed:abstractText
Treatment of peripheral blood lymphocytes from normal donors with small amounts of purified Sendai virions results in enhanced cellular cytotoxicity in vitro to uninfected tissue culture target cells (virus-dependent cellular cytotoxicity (VDCC)), without any obvious correlation to the natural cytotoxicity (NK) displayed by the lymphocytes in the absence of virus. Removal from the virions of the two surface components present in the viral envelope, the HN glycoprotein (gp 71), carrying haemagglutinating and neuraminidase activity, and the F glycoprotein (gp 49), carrying fusion activity, by treatment with pronase abrogated their capacity to induce VDCC. Similar results were obtained when virions lacking the HN glycoprotein after treatment with chymotrypsin were added to the lymphocytes. In contrast, treatment of the virus particles with trypsin, which removed the F glycoprotein, did not affect their capacity to induce VDCC. When the solubilized and separated peplomers were used for lymphocyte treatment, either alone or in combination, the purified HN glycoprotein had full capacity to induce VDCC, whereas the F glycoprotein was inactive. These results suggest that the HM peplomer is solely or primarily responsible for the cytolytic activity arising in non-sensitized lymphocytes when confronted with certain viruses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0300-9475
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-38
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Sendai-virus-induced cell-mediated cytotoxicity in vitro. The role of viral glycoproteins in cell-mediated cytotoxicity.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't