pubmed-article:6300467 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:6300467 | lifeskim:mentions | umls-concept:C0002017 | lld:lifeskim |
pubmed-article:6300467 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
pubmed-article:6300467 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
pubmed-article:6300467 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
pubmed-article:6300467 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:6300467 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:6300467 | pubmed:dateCreated | 1983-5-27 | lld:pubmed |
pubmed-article:6300467 | pubmed:abstractText | Aleutian disease virus (ADV) of mink is a nondefective parvovirus with a single-stranded DNA genome. We characterized the viral DNA forms found in infected cells prepared by a modified Hirt extraction procedure. Double-stranded DNA molecules corresponding in size to 4.8-kilobase-pair duplex monomers and 9.6-kilobase-pair duplex dimers were identified in agarose gels by blot hybridization to 32P-labeled ADV DNA. A rapidly reannealing ADV duplex monomer was isolated on a preparative scale and physically mapped with a series of restriction endonucleases. The map derived was similar to one derived from double-stranded ADV DNA produced by self-primed synthesis on virion DNA, but differed from restriction endonuclease maps reported for other parvovirus DNAs. The purified duplex monomer could be labeled with [32P]dCTP by nick translation and used as a probe in blot hybridization to detect ADV sequences in DNA from small numbers of infected cells. Additional studies indicated that double-stranded ADV DNA could first be detected at 24 h after infection. | lld:pubmed |
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pubmed-article:6300467 | pubmed:language | eng | lld:pubmed |
pubmed-article:6300467 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6300467 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:6300467 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:6300467 | pubmed:month | Mar | lld:pubmed |
pubmed-article:6300467 | pubmed:issn | 0022-538X | lld:pubmed |
pubmed-article:6300467 | pubmed:author | pubmed-author:BloomM EME | lld:pubmed |
pubmed-article:6300467 | pubmed:author | pubmed-author:MayerL WLW | lld:pubmed |
pubmed-article:6300467 | pubmed:author | pubmed-author:GaronC FCF | lld:pubmed |
pubmed-article:6300467 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:6300467 | pubmed:volume | 45 | lld:pubmed |
pubmed-article:6300467 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:6300467 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:6300467 | pubmed:pagination | 977-84 | lld:pubmed |
pubmed-article:6300467 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:6300467 | pubmed:year | 1983 | lld:pubmed |
pubmed-article:6300467 | pubmed:articleTitle | Characterization of the Aleutian disease virus genome and its intracellular forms. | lld:pubmed |
pubmed-article:6300467 | pubmed:publicationType | Journal Article | lld:pubmed |
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