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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1983-5-27
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pubmed:abstractText |
Aleutian disease virus (ADV) of mink is a nondefective parvovirus with a single-stranded DNA genome. We characterized the viral DNA forms found in infected cells prepared by a modified Hirt extraction procedure. Double-stranded DNA molecules corresponding in size to 4.8-kilobase-pair duplex monomers and 9.6-kilobase-pair duplex dimers were identified in agarose gels by blot hybridization to 32P-labeled ADV DNA. A rapidly reannealing ADV duplex monomer was isolated on a preparative scale and physically mapped with a series of restriction endonucleases. The map derived was similar to one derived from double-stranded ADV DNA produced by self-primed synthesis on virion DNA, but differed from restriction endonuclease maps reported for other parvovirus DNAs. The purified duplex monomer could be labeled with [32P]dCTP by nick translation and used as a probe in blot hybridization to detect ADV sequences in DNA from small numbers of infected cells. Additional studies indicated that double-stranded ADV DNA could first be detected at 24 h after infection.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-1062784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-182558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-192696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-225040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-232210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-4130570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-4178323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-4291934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-4979953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-51871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-6086042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-6163374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-6252342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-6264106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-6264109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-6284985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-6287034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-789912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300467-881736
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
977-84
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6300467-Aleutian Mink Disease Virus,
pubmed-meshheading:6300467-Animals,
pubmed-meshheading:6300467-Cats,
pubmed-meshheading:6300467-Cells, Cultured,
pubmed-meshheading:6300467-DNA, Viral,
pubmed-meshheading:6300467-DNA Replication,
pubmed-meshheading:6300467-Genes, Viral,
pubmed-meshheading:6300467-Kidney,
pubmed-meshheading:6300467-Kinetics,
pubmed-meshheading:6300467-Virion,
pubmed-meshheading:6300467-Virus Cultivation,
pubmed-meshheading:6300467-Virus Replication,
pubmed-meshheading:6300467-Viruses, Unclassified
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pubmed:year |
1983
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pubmed:articleTitle |
Characterization of the Aleutian disease virus genome and its intracellular forms.
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pubmed:publicationType |
Journal Article
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