Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-5-27
pubmed:abstractText
A mutant of herpes simplex virus type 1, 17tsVP1201, has a temperature-sensitive processing defect in a late virus polypeptide. Immunoprecipitation studies with monoclonal antibodies showed that the aberrant polypeptide in mutant virus-infected cells was the nucleocapsid polypeptide known as p40. Since a revertant, TS(+) for growth, processed the polypeptide normally under conditions restrictive for the mutant, the processing event must be essential for virus replication. Electron microscopic analysis of mutant virus-infected cells grown at the nonpermissive temperature revealed that the nuclei contained large aggregations of empty nucleocapsids possessing some internal structure. Therefore, although the mutant synthesized virus DNA at the nonpermissive temperature, the DNA was not packaged into nucleocapsids. When mutant virus-infected cells were shifted from 39 to 31 degrees C in the presence of cycloheximide, the polypeptide p40 was processed to lower-molecular-weight forms, and full nucleocapsids were detected in the cell nuclei. The aberrant polypeptide of the mutant, however, was not processed in cells mixedly infected with 17tsVP1201 and a revertant at the nonpermissive temperature, suggesting that the defect of the mutant was in the gene encoding p40 rather than in a gene of a processing enzyme.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-1090574, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-1102604, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-13286423, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-14468055, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-163288, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-181532, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-185412, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-211178, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-212602, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-214583, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-219240, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-220789, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-4117962, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-4129836, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-4316013, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-4344252, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-4348796, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-6154808, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-6158580, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-6159543, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-6245265, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-6262999, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-6268805, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-6278727, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-6280181, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-85720, http://linkedlifedata.com/resource/pubmed/commentcorrection/6300447-91687
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1056-64
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Identification and characterization of a herpes simplex virus gene product required for encapsidation of virus DNA.
pubmed:publicationType
Journal Article