Linked Life Data

6300027

Source:http://linkedlifedata.com/resource/pubmed/id/6300027

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1983-5-5
pubmed:abstractText
Complementary (c)DNA clones of phenobarbital-inducible cytochrome P-450 which has a strong activity toward metabolic activation of aflatoxin B1 were identified by a hybridization-arrested translation assay and a positive hybridization-translation assay. The coding nucleotide sequence of the cytochrome P-450 mRNA was determined by analysis of three cloned cDNAs and by primer extension method using a 5' terminal region fragment (62 bp) of a clone (pcP-450 pb-2) as a primer. The deduced amino acid sequence of the cytochrome is composed of 491 amino acid and its predicted molecular weight and amino acid composition concur with those determined with the purified protein. The sequence of one of the three cloned cDNAs is not completely the same as that of the other two. Fourteen nucleotide substitutions occur in their 922 overlapping nucleotides and 7 of them result in 6 amino acid replacements, therefore indicating the presence of at least two similar but distinct mRNAs for phenobarbital-inducible cytochrome P-450. These substitutions occur in a limited portion of the sequence, apparently forming some sort of a "variable regions."
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:author
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Molecular cloning and coding nucleotide sequence of complementary DNA of cytochrome P-450 involved in metabolic activation of carcinogenic substances.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't