Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1983-5-5
|
pubmed:abstractText |
The locations of thirty restriction endonuclease cleavage sites were determined on the genome of adenovirus type 4 (Ad4), the sole member of the subgroup E adenovirions. The restriction endonucleases Bg/II, EcoRI, HindIII, HpaI, KpnI, SalI, and XbaI cut Ad4 DNA 10, 3, 2, 3, 5, 5 and 3 times, respectively. Orientation of the linear Ad4 map with respect to left and right molecular ends was accomplished by taking advantage of the limited sequence homology between Ad2 and Ad4. Ten non-overlapping fragments of Ad4 DNA representing 98% of the genome, map units 1.6 to 99.6, have been cloned into the plasmid vector pKC7.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0378-1119
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
20
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
205-10
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:6299888-Adenoviridae,
pubmed-meshheading:6299888-Adenoviruses, Human,
pubmed-meshheading:6299888-Adenoviruses, Simian,
pubmed-meshheading:6299888-Base Sequence,
pubmed-meshheading:6299888-Cloning, Molecular,
pubmed-meshheading:6299888-DNA, Viral,
pubmed-meshheading:6299888-DNA Restriction Enzymes,
pubmed-meshheading:6299888-Microscopy, Electron,
pubmed-meshheading:6299888-Plasmids,
pubmed-meshheading:6299888-Species Specificity
|
pubmed:year |
1982
|
pubmed:articleTitle |
Restriction mapping and molecular cloning of adenovirus type 4 (subgroup E) DNA.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|