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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1983-5-27
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pubmed:abstractText |
We have shown that the 34 bp internal control region of the somatic 5S RNA gene from Xenopus borealis can be split into two separable components. A maxigene carrying an insertion between nucleotide 71 and nucleotide 74 of the coding region is actively transcribed in the nucleus of X. laevis oocytes, giving rise to a maxitranscript with initiation and termination points identical with those of the wild-type transcript. The first 11 bases of the 5S RNA gene promoter are shown to be structurally and functionally homologous with the first component (box A) of the promoter for tRNA genes. This was shown by constructing hybrid 5S RNA-tRNAPro and tRNAPro-5S RNA genes that were efficiently transcribed in the X. laevis oocytes. Initiation of transcription appears to be a complex phenomenon in which both components of the internal promoter play a role.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0092-8674
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
725-33
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6299574-Animals,
pubmed-meshheading:6299574-Base Sequence,
pubmed-meshheading:6299574-DNA Restriction Enzymes,
pubmed-meshheading:6299574-DNA-Directed RNA Polymerases,
pubmed-meshheading:6299574-Deoxyribonuclease BamHI,
pubmed-meshheading:6299574-Deoxyribonuclease EcoRI,
pubmed-meshheading:6299574-Operon,
pubmed-meshheading:6299574-RNA, Transfer,
pubmed-meshheading:6299574-RNA Polymerase III,
pubmed-meshheading:6299574-Transcription, Genetic,
pubmed-meshheading:6299574-Xenopus
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pubmed:year |
1983
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pubmed:articleTitle |
Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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