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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1983-4-21
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pubmed:abstractText |
Under the influence of testosterone, rat seminal vesicles synthesise large amounts of a tissue specific protein, S. Recombinant lambda clones have been isolated containing overlapping sequences covering a 27.5 kilo base region of the rat genome within which the gene for protein S is located. Recombinant plasmids bearing cDNA sequences for protein S were constructed in pBR328. One (pcS2) contains a 690 nucleotide insert and is probably full length. Detailed restriction maps of the S-gene are presented and the structure was confirmed by analysis of R-loops and heteroduplexes. The S-gene covers a 2 kbp region of the genome and consists of a 5' intron (490 bp) separating a leading exon (120 bp) containing the 5' untranslated region from a central exon (310 bp) containing most of the coding sequence and part of the 3' untranslated region. A larger intron (1100 bp) lies within the 3' untranslated region. The cloned gene is representative of the native gene but the S gene may be heterogeneous. Using pcS2, the hormonal control of S-specific mRNA was examined and a pronounced differential response to testosterone was observed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-1008845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-158758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-286292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-376855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-440416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-4594039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-479179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-593900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6122202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6164983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6166921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6245241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6248430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6266414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6271628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6290325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6292830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6889996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6935664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6959108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-6993482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-7007263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-708407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-7327300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-7433091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-83232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-845942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-881736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-912752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298734-94421
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Prostatic Secretory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Seminal Plasma Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Testicular Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Testosterone,
http://linkedlifedata.com/resource/pubmed/chemical/beta-microseminoprotein
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
917-30
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6298734-Animals,
pubmed-meshheading:6298734-Castration,
pubmed-meshheading:6298734-Cloning, Molecular,
pubmed-meshheading:6298734-DNA,
pubmed-meshheading:6298734-DNA, Recombinant,
pubmed-meshheading:6298734-DNA Restriction Enzymes,
pubmed-meshheading:6298734-Genes,
pubmed-meshheading:6298734-Male,
pubmed-meshheading:6298734-Microscopy, Electron,
pubmed-meshheading:6298734-Plasmids,
pubmed-meshheading:6298734-Prostatic Secretory Proteins,
pubmed-meshheading:6298734-Proteins,
pubmed-meshheading:6298734-RNA, Messenger,
pubmed-meshheading:6298734-Rats,
pubmed-meshheading:6298734-Rats, Inbred Strains,
pubmed-meshheading:6298734-Seminal Plasma Proteins,
pubmed-meshheading:6298734-Seminal Vesicles,
pubmed-meshheading:6298734-Testicular Hormones,
pubmed-meshheading:6298734-Testosterone
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pubmed:year |
1983
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pubmed:articleTitle |
Isolation and characterisation of genes for androgen-responsive secretory proteins of rat seminal vesicles.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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