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rdf:type |
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lifeskim:mentions |
umls-concept:C0033684,
umls-concept:C0038975,
umls-concept:C0242961,
umls-concept:C0392918,
umls-concept:C0449830,
umls-concept:C1145667,
umls-concept:C1167622,
umls-concept:C1522605,
umls-concept:C2246376,
umls-concept:C2349209,
umls-concept:C2825311
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pubmed:issue |
1
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pubmed:dateCreated |
1983-4-21
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pubmed:abstractText |
DNA binding regions I, II, and III at the origin of replication have different arrangements of A protein (T antigen) recognition pentanucleotides. The A protein also protects each region from DNase in distinctly different patterns. Footprint and fragment assays led to the following conclusions: (i) in some cases a single recognition pentanucleotide is sufficient to direct the binding and accurate alignment of A protein on DNA; (ii) the A protein binds within isolated region I or II in a sequential process leading to multiple overlapping areas of DNase protection within each region; and (iii) the 23-base pair span of recognition sequences in region II allows binding and protection of a longer length of DNA than the 23-base pair span in region I. We propose a model of protein binding that addresses the problem of variations in the arrangement of pentanucleotides in regions I and II and explains the observed DNase protection patterns. The central feature of the model requires each protomer of A protein to bind to a pentanucleotide in a unique direction. The resulting orientation of protein would protect more DNA at the 5' end of the 5'-GAGGC-3' recognition sequence than at the 3' end. The arrangement of multiple protomers at the origin of simian virus 40 replication is discussed.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-172906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-188810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-202398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-212715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-226310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-4343541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6178514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6246927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6248243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6251230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6270901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6270902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6273580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6278740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6281769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6283167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6288963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6292510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6298451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6298452-6461415
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
151-61
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6298452-Antigens, Viral,
pubmed-meshheading:6298452-Antigens, Viral, Tumor,
pubmed-meshheading:6298452-DNA, Viral,
pubmed-meshheading:6298452-DNA Replication,
pubmed-meshheading:6298452-Simian virus 40,
pubmed-meshheading:6298452-Sulfuric Acid Esters,
pubmed-meshheading:6298452-Virus Replication
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pubmed:year |
1983
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pubmed:articleTitle |
Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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