Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1983-3-11
pubmed:abstractText
The unique ability of bovine papillomavirus (BPV-1) DNA to replicate as a stable, multicopy plasmid in transformed mouse cells has led to its utilization as a eukaryotic cloning vector. One limitation of the system has been the marked reduction in transformation efficiency when BPV-1 DNA or the subgenomic transforming segment of BPV-1 DNA (BPV69T) is covalently linked to pBR322 sequences. A dual host replicon consisting of BPV-1 DNA and pML2d, a deletion variant of pBR322, was constructed and shown to be highly efficient for transformation of mouse cells in vitro. The hybrid molecule replicates as a stable, unintegrated, multicopy plasmid in transformed mouse cells. The resident BPV-1-pML2d plasmid DNA was rescued in bacteria and the recovered plasmids were shown to be identical in structure and to have the same transformation efficiency as the original transforming DNA. In contrast, the transforming efficiency of BPV69T DNA is less than 1/100th that of BPV-1 DNA when the DNA is left covalently linked to pML2d. These observations indicate that, although the nontransforming region of BPV-1 (BPV31NT) DNA is not essential for transformation, it has a facilitative role in the transformation process.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-291033, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-4200179, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-4312123, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-4705382, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6100967, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6162401, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6247821, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6250708, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6251381, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6251547, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6253995, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6256739, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6265905, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6267479, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6269067, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6269464, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6280384, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6287462, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-6766891, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-726266, http://linkedlifedata.com/resource/pubmed/commentcorrection/6296820-881736
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7147-51
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Transformation and replication in mouse cells of a bovine papillomavirus--pML2 plasmid vector that can be rescued in bacteria.
pubmed:publicationType
Journal Article