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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1983-3-17
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pubmed:abstractText |
1. The effects of isoprenaline (10(-6) M) on relaxation, unidirectional as well as net Ca(2+) fluxes, and cyclic AMP levels were investigated in rabbit aorta under the condition of high-K(+) depolarization in the presence of phentolamine (10(-5) M).2. Isoprenaline (10(-6) M) caused significant inhibition of Ca(2+) influx stimulated by 145 mM-K(+) (0 Na(+)) solution. The time courses of Ca(2+) influx inhibition and relaxation by isoprenaline were parallel. Isoprenaline also caused a significant inhibition of high-K(+)-induced gain in net Ca(2+) content.3. Ro 20-1724 (1 mM), a phosphodiesterase inhibitor, also caused relaxation and Ca(2+) influx inhibition in high-K(+)-depolarized rabbit aorta. Pre-treatment with Ro 20-1724 potentiated isoprenaline-induced Ca(2+) influx inhibition and relaxation.4. Isoprenaline and Ro 20-1724 each alone increased cyclic AMP levels. Furthermore pre-treatment with Ro 20-1724 caused potentiation of isoprenaline-induced increases in cyclic AMP levels.5. At submaximal concentration, D600 (10(-7) M) caused partial inhibition of high-K(+)-stimulated Ca(2+) influx and produced relaxation. However, unlike Ro 20-1724, it did not potentiate isoprenaline-induced Ca(2+) influx inhibition and relaxation. D600 does not increase cyclic AMP levels in smooth muscle.6. Dibutyryl cyclic AMP (1 mM), a lipid-soluble analogue of cyclic AMP, caused relaxation and inhibited high-K(+)-stimulated Ca(2+) influx.7. Isoprenaline failed to cause stimulation of Ca(2+) efflux in high-K(+)-depolarized rabbit aorta.8. It is concluded that the inhibition of Ca(2+) influx may be one of the mechanisms by which beta-receptor stimulation can reduce intracellular free Ca(2+) to promote relaxation of smooth muscle. The data support the involvement of cyclic AMP in this action of the beta-agonist.9. Since the experiments were conducted in 145 mM-K(+) (0 Na(+)) depolarizing conditions, the role of hyperpolarization or of a Na(+)-Ca(2+) exchange mechanism in isoprenaline-induced Ca(2+) influx inhibition and/or relaxation can be excluded.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-1142092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-196944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-201748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-208382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-220406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-227262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-227273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-233130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-233257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-4144682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-4146202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-4296170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-4367920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-512955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-520410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-5323765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-5684295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-6073560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-6243565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-6249550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-6259328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-7241573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-7320922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-7441538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6296369-810772
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
331
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
429-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6296369-4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone,
pubmed-meshheading:6296369-Animals,
pubmed-meshheading:6296369-Aorta,
pubmed-meshheading:6296369-Biological Transport,
pubmed-meshheading:6296369-Bucladesine,
pubmed-meshheading:6296369-Calcium,
pubmed-meshheading:6296369-Cyclic AMP,
pubmed-meshheading:6296369-Isoproterenol,
pubmed-meshheading:6296369-Muscle, Smooth, Vascular,
pubmed-meshheading:6296369-Muscle Relaxation,
pubmed-meshheading:6296369-Potassium,
pubmed-meshheading:6296369-Rabbits
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pubmed:year |
1982
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pubmed:articleTitle |
Effects of beta-adrenergic stimulation on calcium movements in rabbit aortic smooth muscle: relationship with cyclic AMP.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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