Linked Life Data

6296256

Source:http://linkedlifedata.com/resource/pubmed/id/6296256

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1983-3-11
pubmed:abstractText
The v-src gene was removed from Rous sarcoma virus DNA and replaced with either a cDNA or genomic clone of human alpha-chorionic gonadotropin. Transfection of the recombinant retrovirus genomes into normal chicken fibroblasts produced nontransforming recombinant virus in high titer. Neither helper virus nor selective conditions were needed. Cells infected with the recombinant viruses expressed RNA containing the gonadotropin sequences in levels equivalent to those of term human placenta (approximately 0.5% of poly A+). Essentially every fibroblast in the culture was infected; the infected cells contained approximately one recombinant provirus each. The gonadotropin intervening sequences were removed precisely from the recombinant genomes that contained them, creating recombinants carrying a perfect cDNA copy of the original genomic insert. However, the intervening sequences were removed inefficiently such that several viral replicative cycles were necessary before all genomes had been processed completely. The implications of these observations to the transduction of viral oncogenes and the creation of processed pseudogenes are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0271-6801
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
547-59
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Splicing of intervening sequences introduced into an infectious retroviral vector.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.