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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1983-3-24
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pubmed:abstractText |
Peroxidation of SCN- to OSCN-, catalysed by myeloperoxidase and lactoperoxidase, was studied. The rate of this reaction showed sharp optima between pH 5 and 7.5, the position of which is determined by the concentrations of both SCN- and H2O2. At low pH values, both SCN- and H+ inhibited myeloperoxidase and lactoperoxidase competitively with respect to H2O2. The inhibition constants of SCN- for myeloperoxidase and lactoperoxidase (2 and 6 mM, respectively) are independent of pH. For these enzymes a Ki for H+ of 1 microM was found that corresponded to an ionisable group on the enzymes (pKa = 6) which controls the enzymic activity. A kinetic expression is proposed that explains most of the data. The physiological consequences of the corresponding mechanism are discussed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
709
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
212-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6295491-Animals,
pubmed-meshheading:6295491-Cattle,
pubmed-meshheading:6295491-Female,
pubmed-meshheading:6295491-Humans,
pubmed-meshheading:6295491-Hydrogen-Ion Concentration,
pubmed-meshheading:6295491-Kinetics,
pubmed-meshheading:6295491-Lactoperoxidase,
pubmed-meshheading:6295491-Leukocytes,
pubmed-meshheading:6295491-Milk,
pubmed-meshheading:6295491-Oxidation-Reduction,
pubmed-meshheading:6295491-Peroxidase,
pubmed-meshheading:6295491-Peroxidases,
pubmed-meshheading:6295491-Thiocyanates
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pubmed:year |
1982
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pubmed:articleTitle |
The peroxidation of thiocyanate catalysed by myeloperoxidase and lactoperoxidase.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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