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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1983-2-25
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pubmed:abstractText |
The GAL4 locus encodes a positive regulator of the inducible galactose and melibiose genes of yeast. Using the yeast plasmid vector YEp13, we have cloned GAL4 by complementation of a gal4 mutation. Restriction endonuclease mapping of subclone DNA has delimited the region sufficient for complementation to a 3.2-kilobase segment of DNA. The GAL4 mRNA is 2.8 kilobases long, sufficient to encode a protein as large as 105,000 daltons. The concentration of the GAL4 transcript is about 0.1 per cell and is almost identical in galactose-induced and noninduced cells. This result is consistent with a previously proposed model in which the activity of the GAL4 protein and not the transcription of the GAL4 gene is modulated by galactose induction.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-1055375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-14158615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-183818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-207666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-321129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-327270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-347451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-357984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-369708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-370827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-378392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-385230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-395029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-414220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-6246523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-6276569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-6294669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-6988385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-6990421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294656-7002730
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6827-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6294656-Cloning, Molecular,
pubmed-meshheading:6294656-DNA, Fungal,
pubmed-meshheading:6294656-DNA, Recombinant,
pubmed-meshheading:6294656-DNA Restriction Enzymes,
pubmed-meshheading:6294656-Genes, Regulator,
pubmed-meshheading:6294656-Nucleic Acid Hybridization,
pubmed-meshheading:6294656-Plasmids,
pubmed-meshheading:6294656-RNA, Messenger,
pubmed-meshheading:6294656-Saccharomyces cerevisiae,
pubmed-meshheading:6294656-Transcription, Genetic
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pubmed:year |
1982
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pubmed:articleTitle |
Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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