Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-2-25
pubmed:abstractText
The genes encoding inducible tetracycline resistance in Tn1721 were located in a 2.1-kilobase portion of the transposon. Using deletions and insertions, we mapped and characterized two tet genes by their mutant phenotypes. Two tetracycline-inducible polypeptides synthesized in minicells were assigned to the tet genes. The polarity of the tet genes was determined by employing a deletion and a gene fusion which altered the carboxy termini of the polypeptides. The gene responsible for resistance (tetA) encompasses 1,250 base pairs and encodes a membrane-bound protein with an apparent molecular weight of 34,000. The second gene (tetR) encompasses at least 650 base pairs and encodes a soluble 26,000-dalton protein, identified by complementation analysis as the repressor. The two adjacent genes have opposite transcriptional polarity, suggesting that the sites controlling their expression are located in the intercistronic region between tetA and tetR.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-1267736, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-327263, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-344137, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-374376, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-377024, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-378932, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-390404, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-4564689, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-4612023, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-4896022, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-542128, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6101056, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6252196, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6261087, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6267006, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6267431, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6268611, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6271491, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6278249, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6279565, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6282805, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6285397, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6444453, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6927764, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-6986561, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-7001450, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-7019198, http://linkedlifedata.com/resource/pubmed/commentcorrection/6294046-775491
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
116-23
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Tn1721-encoded tetracycline resistance: mapping of structural and regulatory genes mediating resistance.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't