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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1982-6-21
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pubmed:abstractText |
It is shown that liposomes containing (i) a fluorescein-labeled murine histocompatibility antigen (FITC-H-2Kk) and the G protein of vesicular stomatitis virus or (ii) H-2Kk and fluorescein-labeled viral protein (FITC-G) can elicit H-2-restricted syngeneic antiviral cytotoxic T cells as assayed by 51Cr release from appropriate virus-infected target cells. Fluorescence recovery after photobleaching was used to measure the diffusion coefficients of these reconstituted proteins in four different samples: (i) FITC-H-2Kk; (ii) FITC-H-2Kk and G; (iii) FITC-G; and (iv) FITC-G and H-2Kk. The same rate of lateral diffusion (D = 1 x 10(-8) cm2/sec at 37 degrees C in 25% cholesterol/75% dimyristoylphosphatidylcholine) was obtained in every case. Both proteins, fluorescent as well as nonfluorescent, could be patched by using specific antibodies. When G was patched with antibody, FITC-H-2Kk did not copatch. When H-2Kk was patched with antibody FITC-G did not copatch. These diffusion and patching measurements rule out the possibility that these proteins have either extensive oligomeric associations or strong specific pairwise associations.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-1059096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-14450081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-204941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-214512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-218577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-221897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-227980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-228303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-291895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-293667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-305464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-349081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-4308915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-448095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-479148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-567555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-61285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-6247354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-6247397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-624903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-6256440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-6257559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-629935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-6893805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-6893854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-6935669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-6970145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-6972537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-7400149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-7459346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6280187-76660
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1506-10
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6280187-Animals,
pubmed-meshheading:6280187-Antigen-Antibody Reactions,
pubmed-meshheading:6280187-Cell Line,
pubmed-meshheading:6280187-Cell Membrane,
pubmed-meshheading:6280187-Diffusion,
pubmed-meshheading:6280187-H-2 Antigens,
pubmed-meshheading:6280187-Liposomes,
pubmed-meshheading:6280187-Membrane Fluidity,
pubmed-meshheading:6280187-Membrane Proteins,
pubmed-meshheading:6280187-Mice,
pubmed-meshheading:6280187-T-Lymphocytes,
pubmed-meshheading:6280187-Vesicular stomatitis Indiana virus,
pubmed-meshheading:6280187-Viral Proteins
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pubmed:year |
1982
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pubmed:articleTitle |
H-2Kk and vesicular stomatitis virus G proteins are not extensively associated in reconstituted membranes recognized by T cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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