Linked Life Data

6278578

Source:http://linkedlifedata.com/resource/pubmed/id/6278578

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1982-5-12
pubmed:abstractText
The binding of 1,25(OH)2 vitamin D3 to its chick duodenal cytosol receptor protein has been analysed on a law of mass action model. Within the limits of experimental error there is such good agreement, when only one binding site is considered, that it is possible to fit the standard assay curve for practical use. Simple pocket-calculator methods are given for this. The equilibrium constant for the reaction, under the conditions used (pH 7.4, 25 degrees C), was 2.1 X 10(10) 1 X mol-1. A kinetic analysis under the same conditions yielded forward and backward reaction rate constants of 6.5 X 10(6) mol-1 X s-1 X l(2) and 3.1 X 10(-4) s-1 X 1, respectively. The kinetic curve also very closely fitted to the experimental data on the basis of the model. The theory for use of the mass action model to optimize the binding-site concentration to give a minimum absolute error for any measured ligand concentration is presented. The use of the model in assay design, standard curve fitting, quality control and problem solving are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0036-5513
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
543-50
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Application of the law of mass action to design and performance of receptor assays for 1,25(OH)2 vitamin D.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't