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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1982-5-21
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pubmed:abstractText |
The apparent molal adiabatic compressibilities of ferri- and ferrocytochrome c have been determined from measurements of density and sound velocity. The values found were +2.99 X 10(-8) and -2.40 X 10(-8) cm5 mol-1 dyne-1 for the ferri and ferro forms, respectively. Experiments were performed on identical solutions containing either the oxidized or reduced form of protein. Solutions of ferricytochrome c were found to have significantly greater adiabatic compressibility than equivalent solutions of ferrocytochrome c at 25 degrees C and pH 7.15. The remarkable similarity of the three-dimensional structures of the ferri and ferro proteins [Takano, T. & Dickerson, R.E. (1980) Proc. Natl. Acad. Sci. USA 77, 6371-6375] strongly suggests that this difference in compressibility is due to an increase in volume fluctuations within ferricytochrome c relative to the ferro form rather than a change in equilibrium structure or hydration. Such a difference in the dynamic properties of the structures is consistent with both the crystallographic thermal B factors and the observed increase in amide hydrogen exchange kinetics when ferrocytochrome c is oxidized. The relative magnitude of the root mean square volume fluctuations is approximated from an ideal solution treatment of the compressibility data and yields a ratio of delta Vrms (ferri cyt c)/ delta Vrms (ferro cyt c) = 1.3.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-1066687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-13897582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-14040866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-189301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-194885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-196283,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-208522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-215917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-38741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-4349479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-435457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-4368360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-4407622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-5119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-5333288,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-5389438,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-6256733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-6279868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-803252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6278497-932018
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
815-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1982
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pubmed:articleTitle |
Increase in apparent compressibility of cytochrome c upon oxidation.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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