Linked Life Data

6276418

Source:http://linkedlifedata.com/resource/pubmed/id/6276418

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-4-12
pubmed:abstractText
Flat variant clones were isolated from both the N18 neuroblastoma cell line and from a subclone of the parent line exhibiting the typical round cell morphology. Several revertant clones exhibiting the round parental morphology were also isolated from one of the flat variant clones. The flat variants exhibit decreased cloning efficiency in suspension and increased amounts of myosin heavy chain when compared to the round cell clones. Intracellular cAMP levels were increased from two- to fivefold over those in clones representative of the parent line and in the round cell revertants. Treatment with the phosphodiesterase inhibitor RO20-1724 increased cAMP levels and reduced suspension cloning efficiency without altering doubling time or attached cloning efficiency. Increasing cAMP levels of two of the round cell clones by treatment with the phosphodiesterase inhibitor caused increased flattening of the cell body and increased myosin heavy chain content. Thus, even though increased cAMP level may be sufficient to explain the reduced cloning efficiency of the flat variants, it is not the sole cause of the flat morphology.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-51
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Clonal variation in cultured neuroblastoma cells. II. The relationship of increased intracellular cyclic AMP content to increased anchorage requirement for growth and flattened morphology.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.