Linked Life Data

6274910

Source:http://linkedlifedata.com/resource/pubmed/id/6274910

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1982-3-13
pubmed:abstractText
We have stimulated in a cultured hepatocyte system the synergistic interaction between triiodothyronine (T3) and dietary carbohydrate in the induction of malic enzyme (ME). Kinetic studies revealed that isolated hepatocytes equilibrate with media T3 within 5 min; nuclei equilibrate with media T3 by 45 min; and the half-time of T3 metabolism was 10 h in 10% serum. We demonstrated nuclear T3 receptors in isolated hepatocytes and the induction of ME by T3 in physiological concentrations. However, in the complete absence of T3 glucose could still induce ME. At all concentrations of glucose (100-1,000 mg/dl), T3 (0.3 nM free T3) resulted in a relatively constant (1.4- to 1.7-fold) increase in ME response. The augmentation in ME activity was paralleled by an enhanced rate of enzyme synthesis as determined by [3H]leucine incorporation into immunoprecipitable ME. Cells cultured in serum free media also demonstrated a glucose-dependent increase in ME. Insulin greatly stimulated the glucose induction of ME, whereas dexamethasone had very little influence on ME induction. These studies demonstrate the usefulness of an adult hepatocyte tissue culture model for the study of the effects of T3 on gene expression in cells that are not derived from tumor. They clearly demonstrate that well established effects of T3 can be simulated in such a system at levels of free hormone that approximate those in extracellular body fluids. Our results indicate that an increased concentration of glucose per se can induce the formation of ME in the absence of alterations in extrahepatic hormones or factors. Moreover, our findings confirm inferences from in vivo studies that T3 acts as a multiplier of a glucose-induced signal.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-129545, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-14083167, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-177845, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-221206, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-221272, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-243, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-4274563, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-4369384, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-446419, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-4812438, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-4900611, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-6995793, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-7240417, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-7356948, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-807593, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-85301, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-944696, http://linkedlifedata.com/resource/pubmed/commentcorrection/6274910-993859
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1485-90
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Glucose and triiodothyronine both induce malic enzyme in the rat hepatocyte culture: evidence that triiodothyronine multiplies a primary glucose-generated signal.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.