Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1982-2-12
pubmed:abstractText
A 55-kilodalton (kDal) protein was detected recently in primary cultures of day 12 mouse embryos by immunoprecipitation with serum from simian virus 40 (SV40) tumor-bearing hamsters (T serum), Preliminary evidence suggested that this protein was similar to a cellular 55-kDal protein induced after SV40 transformation of mouse cells. We now show that specific approximately 55-kDal [35S]methionine-labeled proteins precipitate from primary cultures of midgestation mouse, rat, and hamster embryos on addition of T serum or monoclonal antiserum prepared against the SV40-induced mouse 55-kDal proteins. The two-dimensional maps of the [35S]methionine-labeled tryptic peptides of the mouse, hamster, and rat embryo proteins are similar to the maps of the corresponding proteins from SV40-transformed cells. Primary cells from midgestation mouse, hamster, or rat embryos contain one-third to one-half as much 55-kDal protein as a SV40-transformed mouse fibroblast cell and nearly the same amount as F9 mouse embryonal carcinoma cells. The amount of 55-kDal protein is greatly reduced on replating the mouse, rat, or hamster embryo primary cells. The amount of this protein in mouse embryos is dependent on the stage of the embryo. The embryo proteins are phosphoproteins.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-1083816, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-162906, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-184963, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-209027, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-218111, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-221923, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-222051, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-222475, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-223058, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-225565, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-225566, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-227604, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-228475, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-396115, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-427789, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6154659, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6155477, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6158579, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6169662, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6244576, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6247503, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6253135, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6253675, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6256642, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6260793, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6264107, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6264441, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6265926, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6933048, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273897-6940183
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6953-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Quantitation and characterization of a species-specific and embryo stage-dependent 55-kilodalton phosphoprotein also present in cells transformed by simian virus 40.
pubmed:publicationType
Journal Article