Linked Life Data

6273873

Source:http://linkedlifedata.com/resource/pubmed/id/6273873

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1982-2-12
pubmed:abstractText
Human low density lipoprotein (LDL) was incubated with an established line of rabbit aortic endothelial cells. Density gradient fractionation showed a time-, concentration-, and temperature-dependent increase in the average density of the LDL (from about 1.036 to as high as 1.070 g/ml). Incubation without cells or with other types of cultured cells (fibroblasts, hepatocytes, 3T3-L1 cells) caused no significant change in density. 125I-Labeled LDL (125I-LDL) recovered after incubation with endothelial cells (EC-modified LDL) was taken up and degraded 3 to 4 times more rapidly than control LDL by resident mouse peritoneal macrophages and by an established tumor line of mouse macrophages (J774 cells). Macrophage degradation of EC-modified 125I-LDL exhibited saturation kinetics (greater than 85% inhibited by excess unlabeled EC-modified LDL). Degradation was also inhibited by unlabeled acetylated LDL and, conversely, unlabeled EC-modified LDL inhibited degradation of acetylated 125I-LDL. Incubation of LDL with conditioned medium-removed from endothelial cell cultures modified neither its density nor its rate of degradation by macrophages. These studies show that endothelial cells have the potential to metabolically modify the LDL molecule, generating a form that is more rapidly degraded by macrophages and that is recognized by the macrophage receptor for acetylated LDL. This process may play a significant role in the pathogenesis of atherosclerosis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-1089721, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-123775, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-1259145, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-177050, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-179091, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-181058, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-184464, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-194923, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-208629, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-218198, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-224077, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-270722, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-4176473, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-4366526, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-491545, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-6260882, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-6260883, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-6262767, http://linkedlifedata.com/resource/pubmed/commentcorrection/6273873-6769124
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6499-503
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Enhanced macrophage degradation of low density lipoprotein previously incubated with cultured endothelial cells: recognition by receptors for acetylated low density lipoproteins.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.