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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1982-1-9
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J02288,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J02290,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J02291,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J02292,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K00932,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K00997,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K01041,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K01071,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K01072,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/V01117,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/V01147
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pubmed:abstractText |
Deletions of polyoma virus DNA around the region that codes for the C-terminus of the viral middle T-antigen were created using a transforming fragment (BamH I/EcoR I) of viral DNA cloned in the plasmid vector pAT153. These species were recloned and assayed for their ability to transform Rat-1 cells in culture. Our results showed that whereas the DNA sequence between the presumed translational termination codon for the viral middle T-antigen and the single viral EcoR I site could be removed with no apparent effect on transformation, the removal of the termination codon itself or any amino acid coding sequences of this protein caused a drastic decrease in the transforming ability of the DNA. Transfection of Rat-1 cells with plasmids that contained viral DNA with deletions which corresponded to the last fourteen or more amino acids of the middle T-antigen never gave rise to cellular transformation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-1178526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-14338226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-186787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-206004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-222480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-225038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-225042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-225866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-226975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-228075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-228249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-358147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-358200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-4134493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-4318787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-4353950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-4365585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6243401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6243821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6246489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6251451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6253128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6253141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6253165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6253998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6254006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6255083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-6258800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6272223-73183
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral, Tumor,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease BamHI,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease EcoRI
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2055-73
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6272223-Amino Acid Sequence,
pubmed-meshheading:6272223-Animals,
pubmed-meshheading:6272223-Antigens, Neoplasm,
pubmed-meshheading:6272223-Antigens, Viral,
pubmed-meshheading:6272223-Antigens, Viral, Tumor,
pubmed-meshheading:6272223-Base Sequence,
pubmed-meshheading:6272223-Cell Transformation, Viral,
pubmed-meshheading:6272223-Cells, Cultured,
pubmed-meshheading:6272223-Cloning, Molecular,
pubmed-meshheading:6272223-DNA, Viral,
pubmed-meshheading:6272223-DNA Restriction Enzymes,
pubmed-meshheading:6272223-Deoxyribonuclease BamHI,
pubmed-meshheading:6272223-Deoxyribonuclease EcoRI,
pubmed-meshheading:6272223-Escherichia coli,
pubmed-meshheading:6272223-Plasmids,
pubmed-meshheading:6272223-Polyomavirus,
pubmed-meshheading:6272223-Rats
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pubmed:year |
1981
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pubmed:articleTitle |
Requirement for the C-terminal region of middle T-antigen in cellular transformation by polyoma virus.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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