Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 1
|
| pubmed:dateCreated |
1981-12-21
|
| pubmed:abstractText |
Epstein-Barr virus (EBV) was labelled with 3H-thymidine and purified about 1000-fold from the culture medium by ultracentrifugation on 5 to 30% dextran gradients. The presence of the virus was monitored by radioactivity and Epstein-Barr virus-determined nuclear antigen (EBNA) induction in sensitive indicator cells (Ramos). Peaks for both activities occurred in the 17 to 18% dextran fractions. Unlabelled virus recovered in the peak fraction was labelled with 125I. Both thymidine and 125I-labelled purified virus bound quantitatively to receptor-positive Burkitt lymphoma-derived cell lines but not to EBV-receptor-negative T-lymphocyte-derived cell lines. Thymidine-labelled virus that was allowed to bind to Raji cells was present in the interior of briefly trypsinized cells after 3 h incubation at 37 degrees C. The results provide a convenient method for detecting the EBV receptor by radioactively labelled virus.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1317
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
54
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
191-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1981
|
| pubmed:articleTitle |
The detection of Epstein-Barr virus receptors utilizing radiolabelled virus.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|