Linked Life Data

6268658

Source:http://linkedlifedata.com/resource/pubmed/id/6268658

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-11-18
pubmed:abstractText
We have adapted the Beckman Airfuge air turbine ultracentrifuge and the new EM-90 particle-counting rotor to improve detection by electron microscopy of viruses in clinical specimens. Samples were clarified by centrifugation, pelleted in the EM-90 rotor directly to Formvar-coated copper grids, and strained with 1.5% sodium phosphotungstate. Virus counts and endpoint titrations of serial dilutions of partially purified preparations of poliovirus, SA11 rotavirus, herpes simplex virus, and vaccinia virus showed an increase of ca. 1.5 log10 to 3.0 log10 over the virus titers of unconcentrated preparations of the same material. An increased yield of 14% more positive specimens for rotavirus was obtained after preparation of clinical samples by direct ultracentrifugation versus a method without virus concentration (82 versus 72). A prospective study showed that detection of adenoviruses, herpesviruses, and enteroviruses increased when specimens were prepared by direct ultracentrifugation. Direct ultracentrifugation with the EM-90 rotor in the Airfuge ultracentrifuge is a rapid concentration method which enhances the rate and yield of virus detection from clinical specimens by electron microscopy and is easily adaptable to a diagnostic virology laboratory.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-166309, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-213712, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-39115, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-4138653, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-4180813, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-6104011, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-6155006, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-6155007, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-6244887, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-6250692, http://linkedlifedata.com/resource/pubmed/commentcorrection/6268658-7207047
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
210-21
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Improved detection of viruses by electron microscopy after direct ultracentrifuge preparation of specimens.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't