Linked Life Data

6267459

Source:http://linkedlifedata.com/resource/pubmed/id/6267459

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-10-25
pubmed:abstractText
Bacteria mutant at the rep gene (specifying a DNA-unwinding enzyme) were slightly more sensitive than rep+ bacteria to far ultraviolet light (ca. 254 nm; (FUV) and to monofunctional psoralen photoproducts produced with near ultraviolet light (ca. 360 nm; NUV). The enhanced sensitivity was shown in uvrA excision-deficient bacteria but not in those carrying the recA mutation. It is concluded that the rep unwinding enzyme has a small promoting effect on post-replication recombination repair. Rep- bacteria were rather more sensitive to the DNA cross-linking action f 8-methoxypsoralen (8-MOP) and NUV. This effect also was found in uvrA but not in recA bacteria. A cross-link repair pathway, dependent on both the recA+ and rep+ genes, therefore exists in addition to the pathway which depends upon the uvrAB endonuclease. Various lines of evidence suggest that the pathway operates at or after DNA replication. Exposure of rep+ bacteria to 8-MOP plus NUV did not induce any activity capable of repairing psoralen cross-links in infecting lambda phage. A possible model involving removal of the cross-link by glycosylase action at the replication fork is proposed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-38
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Influence of mutations at the rep gene on survival of Escherichia coli following ultraviolet light irradiation or 8-methoxypsoralen photosensitization: evidence for a recA+ rep+-dependent pathway for repair of DNA crosslinks.
pubmed:publicationType
Journal Article