|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1981-10-25
|
|
pubmed:abstractText |
To evaluate the effects of glucocorticoids on the Na-K pump in human subjects, were evaluated the intracellular sodium and potassium, 42K influx across and the [3H]ouabain binding to cell membranes of intact human erythrocytes from a group of subjects taking glucocorticoids and a group of normal subjects. Intracellular sodium concentration was lower (7.2 +/- 0.4 vs. 10.9 +/- 0.2 mmol/liter cell water) and intracellular potassium concentration higher (149.8 +/- 1.5 vs. 137.2 +/- 1.2 mmol/liter cell water) in erythrocytes from steroid-treated patients. In spite of a significantly decrease intracellular sodium which normally diminishes ouabain-sensitive 42K influx, the ouabain-sensitive K influx was unchanged in erythrocytes from the steroid-treated group. Maximum [3H]ouabain binding was markedly higher in the steroid-treated group (835 +/- 44 vs. 449 +/- 11 sites/cell). There was close linear correlation between [3H]ouabain binding and inhibition of K pump, suggesting the specificity of ouabain binding to Na-K pump sites on the cell membrane. Association kinetics for ouabain were similar in the two groups despite the marked difference in the amount of [3H]ouabain binding. External potassium concentration required for half-maximum ouabain-sensitive K influx was identical in the two groups. Thus, the additional Na-K pump sites in the steroid-treated group were qualitatively similar to those in normals. These results suggest that administration of glucocorticoids leads to an increase in the number of Na-K pump sites. The increase in the number of Na-K pump sites may explain the low levels of intracellular sodium and higher cell potassium observed in steroid-treated subjects.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-1148392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-1195342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-123185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-123724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-125547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-125764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-127037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-13799836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-14275413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-4244236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-4260019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-4260663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-4262774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-4530695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-4817354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-5079762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-5411480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-5476387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-5547272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-6018751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-6033588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-6050859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-683206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-722573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-722574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-756487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6267104-864005
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0021-9738
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
68
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
422-30
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:6267104-Adult,
pubmed-meshheading:6267104-Aged,
pubmed-meshheading:6267104-Erythrocyte Aging,
pubmed-meshheading:6267104-Erythrocyte Membrane,
pubmed-meshheading:6267104-Erythrocytes,
pubmed-meshheading:6267104-Female,
pubmed-meshheading:6267104-Glucocorticoids,
pubmed-meshheading:6267104-Humans,
pubmed-meshheading:6267104-Male,
pubmed-meshheading:6267104-Methylprednisolone Hemisuccinate,
pubmed-meshheading:6267104-Middle Aged,
pubmed-meshheading:6267104-Ouabain,
pubmed-meshheading:6267104-Potassium,
pubmed-meshheading:6267104-Sodium,
pubmed-meshheading:6267104-Sodium-Potassium-Exchanging ATPase
|
|
pubmed:year |
1981
|
|
pubmed:articleTitle |
Glucocorticoid-induced alterations in the sodium potassium pump of the human erythrocyte.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
