rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
4
|
pubmed:dateCreated |
1981-8-20
|
pubmed:abstractText |
The integrated proviral genome of Abelson murine leukemia virus (A-MuLV) was cloned in lambda gtWES . lambda B bacteriophage after EcoRI endonuclease digestion and enrichment of proviral sequences by sequential RPC-5 column chromatography and agarose gel electrophoresis. Recombinant DNA clones containing a 7.8-kilobase-pair EcoRI insert were shown to have the entire integrated A-MuLV genome with both 5' and 3' ends flanked by mink cellular DNA sequences. This DNA fragment was shown to induce focus transformation upon transfection of NIH/3T3 mouse cells. Moreover, focus-forming virus could be rescued from transformed nonproducer cells upon superinfection with a type C helper virus. A polyprotein of molecular weight 120,000 (p120) containing murine leukemia virus gag gene determinants was invariably deteced by immunoprecipitation analysis of individual transformants induced by the 7.8-kilobase-pair DNA. Molecularly cloned integrated A-MuLV in its infectious form should be of use in elucidating the mechanisms involved in transformation by this virus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-159456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-163444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-168584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-183818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-209468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-214586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-224582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-229975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-270684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-279004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-338419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-392518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-4311790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-4318987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-4918190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-503218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-608592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6154148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6244493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6248244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6251463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6251547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6257398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-719759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-787937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-88264
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pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0027-8424
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
78
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2077-81
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
pubmed-meshheading:6264461-Abelson murine leukemia virus,
pubmed-meshheading:6264461-Animals,
pubmed-meshheading:6264461-Bacteriophage lambda,
pubmed-meshheading:6264461-Cell Line,
pubmed-meshheading:6264461-Cell Transformation, Viral,
pubmed-meshheading:6264461-Cloning, Molecular,
pubmed-meshheading:6264461-DNA, Viral,
pubmed-meshheading:6264461-DNA Restriction Enzymes,
pubmed-meshheading:6264461-Defective Viruses,
pubmed-meshheading:6264461-Gene Expression Regulation,
pubmed-meshheading:6264461-Genes, Viral,
pubmed-meshheading:6264461-Leukemia Virus, Murine,
pubmed-meshheading:6264461-Mice,
pubmed-meshheading:6264461-Virus Replication
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pubmed:year |
1981
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pubmed:articleTitle |
Abelson murine leukemia virus: molecular cloning of infectious integrated proviral DNA.
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pubmed:publicationType |
Journal Article
|