Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1981-8-20
pubmed:abstractText
The integrated proviral genome of Abelson murine leukemia virus (A-MuLV) was cloned in lambda gtWES . lambda B bacteriophage after EcoRI endonuclease digestion and enrichment of proviral sequences by sequential RPC-5 column chromatography and agarose gel electrophoresis. Recombinant DNA clones containing a 7.8-kilobase-pair EcoRI insert were shown to have the entire integrated A-MuLV genome with both 5' and 3' ends flanked by mink cellular DNA sequences. This DNA fragment was shown to induce focus transformation upon transfection of NIH/3T3 mouse cells. Moreover, focus-forming virus could be rescued from transformed nonproducer cells upon superinfection with a type C helper virus. A polyprotein of molecular weight 120,000 (p120) containing murine leukemia virus gag gene determinants was invariably deteced by immunoprecipitation analysis of individual transformants induced by the 7.8-kilobase-pair DNA. Molecularly cloned integrated A-MuLV in its infectious form should be of use in elucidating the mechanisms involved in transformation by this virus.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-159456, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-163444, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-168584, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-183818, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-209468, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-214586, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-224582, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-229975, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-270684, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-279004, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-322279, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-338419, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-392518, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-4311790, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-4318987, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-4705382, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-4918190, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-503218, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-608592, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6154148, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6244493, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6248244, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6251463, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6251547, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-6257398, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-719759, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-787937, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264461-88264
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2077-81
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Abelson murine leukemia virus: molecular cloning of infectious integrated proviral DNA.
pubmed:publicationType
Journal Article