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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1981-8-20
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pubmed:abstractText |
Thyrotropin-releasing hormone (TRH) binds to specific receptors on GH4C1 pituitary tumor cells. A fluorescently labeled analog of TRH was synthesized by coupling pGlu-His-ProNH(CH2)6NH2 to fluorescein isothiocyanate. The fluorescein-labeled peptide (FL-TRH) stimulated prolactin synthesis and release by GH4C1 cultures and bound to TRH receptors with an apparent Kd of 400 nM. Binding of FL-TRH to unfixed, viable GH4C1 cells was followed by fluorescence microscopy. After incubation with 1.4 microM FL-TRH for 1 hr at 37 degrees C, the surface of all cells was fluorescent and patches of intense fluorescence were evident. Control cultures incubated with FL-TRH and excess TRH were not fluorescent, and a line of pituitary tumor cells which lacks TRH receptors displayed little fluorescence after incubation with FL-TRH. When GH4C1 cells were incubated with FL-TRH for 1 hr at 37 degrees C and then with excess TRH for an additional 1 hr, the fluorescence associated with the cells was diminished to control levels. The results demonstrate that the fluorescein-labeled peptide labels specific TRH receptors.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-15499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-169122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-169886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-173314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-180421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-208640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-209028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-227058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-28075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-307249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-356052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-4008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-412845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-416366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-4209271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-4212470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-4353634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-4364413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-4621548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-4951281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-6243545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264450-80283
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
587-91
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:6264450-Animals,
pubmed-meshheading:6264450-Cell Line,
pubmed-meshheading:6264450-Fluoresceins,
pubmed-meshheading:6264450-Microscopy, Fluorescence,
pubmed-meshheading:6264450-Pituitary Neoplasms,
pubmed-meshheading:6264450-Prolactin,
pubmed-meshheading:6264450-Rats,
pubmed-meshheading:6264450-Receptors, Cell Surface,
pubmed-meshheading:6264450-Receptors, Thyrotropin-Releasing Hormone,
pubmed-meshheading:6264450-Thyrotropin-Releasing Hormone
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pubmed:year |
1981
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pubmed:articleTitle |
Direct visualization of receptors for thyrotropin-releasing hormone with a fluorescein-labeled analog.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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