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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1981-8-20
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pubmed:abstractText |
A hybrid plasmid (pAGO) that contains the herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene in the form of a 2-kilobase-pair (kbp) Pvu II fragment inserted at the Pvu II site of plasmid pBR322 was used to transform TK- Escherichia coli K-12 strain KY895. pAGO-transformed KY895 cells exhibited partially restored ability to incorporate [3H]dThd into DNA and an HSv-1-specific TK activity. Bacteria cured of plasmid pAGO (or transformed by plasmid pBR322) did not show enhanced incorporation of [3H]dThd into DNA or HSV-1 TK activity. Plasmid pMH1A was derived from pAGO by deletion of 2067 bp of DNA sequence from pBR322 and 105 bp from the HSV-1 TK gene. E. coli K-12 strain KY895 cells transformed by pMH1A did not show enhanced incorporation of [3H]dThd into bacterial DNA, although pMH1A DNA isolated from transformed KY895 cells, like pAGO DNA, did transform TK- mouse fibroblast [LM(TK-)] cells to the TK+ phenotype. The expression of HSV-1 TK activity by E. coli K-12 suggests that intervening sequences may be absent from the coding region of HSV-1 tk or that the coding region of the gene possesses short intervening sequences which do not disrupt the translational reading frame.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-100226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-112912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-14033128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-14056989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-14114853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-14114854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-143663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-16590598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-201586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-226978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-231664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-322128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-344137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-354690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-360074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-373120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-383387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-383580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-4228236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-4324302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-4868676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-4922220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-4966087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-5719216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-6154671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-6251599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-6445419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264449-6987524
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
582-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6264449-Animals,
pubmed-meshheading:6264449-DNA, Recombinant,
pubmed-meshheading:6264449-DNA, Viral,
pubmed-meshheading:6264449-Electrophoresis, Agar Gel,
pubmed-meshheading:6264449-Escherichia coli,
pubmed-meshheading:6264449-Genes,
pubmed-meshheading:6264449-Genes, Viral,
pubmed-meshheading:6264449-L Cells (Cell Line),
pubmed-meshheading:6264449-Mice,
pubmed-meshheading:6264449-Plasmids,
pubmed-meshheading:6264449-Simplexvirus,
pubmed-meshheading:6264449-Thymidine,
pubmed-meshheading:6264449-Thymidine Kinase,
pubmed-meshheading:6264449-Transformation, Genetic
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pubmed:year |
1981
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pubmed:articleTitle |
Herpes simplex virus thymidine kinase activity of thymidine kinase-deficient Escherichia coli K-12 mutant transformed by hybrid plasmids.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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