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rdf:type |
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lifeskim:mentions |
umls-concept:C0020792,
umls-concept:C0035696,
umls-concept:C0038975,
umls-concept:C0086860,
umls-concept:C0150305,
umls-concept:C0449432,
umls-concept:C0733755,
umls-concept:C1179435,
umls-concept:C1314939,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1561964,
umls-concept:C1705248
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pubmed:issue |
1
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pubmed:dateCreated |
1981-8-20
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pubmed:databankReference |
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pubmed:abstractText |
The 5' termini of the principal early mRNAs produced in cells transformed by wild-type simian virus 40 lie 21-25 nucleotides downstream from an A-T-T-T-A-T sequence on the DNA template. The 5' termini of early mRNAs produced by five origin-defective mutants containing deletions downstream from the A-T-T-T-A-T sequence and one viable mutant dl892 with a deletion starting 15 nucleotides upstream from this sequence were determined by a method involving synthesis, separation, and determination of the sequences of DNAs complementary to 5' termini. Mutant dl892 produced early mRNAs with the same principal 5' termini as wild-type virus; the origin-defective mutants produced mRNAs with principal 5' termini shifted downstream by a distance equivalent to the length of the deleted DNA segment. These data suggest that a DNA sequence of 29 nucleotides, which includes the A-T-T-T-A-T sequence, contains a component(s) of a promoter for early transcription. This component functions in positioning the 5' ends of the principal early mRNAs 21-25 nucleotides downstream from the A-T-T-T-A-T sequence and acts independently of these downstream sequences.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-166203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-184459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-196100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-202398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-205802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-205947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-206891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-214761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-218021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-222458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-226954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6154870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6159134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6243777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6248238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6252349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6253143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6253995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6254000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-6929494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-7357599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-7383156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-90157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264425-94251
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
100-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6264425-Base Sequence,
pubmed-meshheading:6264425-DNA, Viral,
pubmed-meshheading:6264425-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6264425-Gene Expression Regulation,
pubmed-meshheading:6264425-Genes, Synthetic,
pubmed-meshheading:6264425-Mutation,
pubmed-meshheading:6264425-Operon,
pubmed-meshheading:6264425-RNA, Messenger,
pubmed-meshheading:6264425-RNA, Viral,
pubmed-meshheading:6264425-Simian virus 40,
pubmed-meshheading:6264425-Transcription, Genetic
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pubmed:year |
1981
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pubmed:articleTitle |
Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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