Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-8-10
pubmed:abstractText
We have prepared full-length DNA clones of the Abelson murine leukemia virus (A-MuLV) genome. A specific probe homologous to the central portion of the A-MuLV genome was prepared by nick translation of a subcloned restriction fraction from the cloned DNA. The probe was used to examine the genome structure of several A-MuLV variants. The conclusions are: (i) three viruses coding for Abelson-specific proteins of molecular weight 120,000, 100,000, and 90,000 had genomes indistinguishable in size, suggesting that the shorter proteins are the result of early translational termination; (ii) compared with the genome encoding the 120,000-dalton (120K) protein, a genome coding for a 160K protein was 0.8 kilobase larger in the A-MuLV-specific region; and (iii) a genome coding for a 92K protein had a 700-base pair deletion internal to the coding region. This mutant was transformation defective: its 92K protein lacked the protein kinase activity normally associated with the A-MuLV protein, and cells containing the virus were not morphologically transformed. In addition, we determined the number of A-MuLV proviruses in each of several transformed fibroblast and lymphoid cells prepared by infection in vitro. These experiments show that a single copy of the A-MuLV provirus is sufficient to transform both types of cells and that nonproducer cells generally have only one integrated provirus.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-163444, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-178822, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-206646, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-209468, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-211510, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-215325, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-291033, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-4318922, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-4355680, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-4918190, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-6243819, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-6244493, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-6245237, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-6246487, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-6254050, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-6254060, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-6257398, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-6257926, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-728982, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-80281, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-881736, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-90175, http://linkedlifedata.com/resource/pubmed/commentcorrection/6264122-90344
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
460-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Genome structure of Abelson murine leukemia virus variants: proviruses in fibroblasts and lymphoid cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't