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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1981-8-10
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pubmed:abstractText |
Wheat germ contains an enzyme capable of removing supercoils from circular DNA. We have purified this enzyme using Polymin P fractionation, ammonium sulfate precipitation, and chromatography on Bio-Rex 70 and phenyl-Sepharose. Renaturation after electrophoresis on sodium dodecyl sulfate-polyacrylamide gels shows that topoisomerase activity is associated with a polypeptide with a Mr = about 111,000. The enzyme is similar to other eukaryotic type I DNA topoisomerases (nicking-closing enzymes) by the following criteria: it is capable of increasing or decreasing the topological linking number of covalently closed DNA substrate; it is capable of restoring an equilibrium distribution of linking numbers to DNA substrate with a single unique linking number; and it does not require magnesium ion or ATP for activity.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
256
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5860-5
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6263892-Cations, Divalent,
pubmed-meshheading:6263892-Cations, Monovalent,
pubmed-meshheading:6263892-DNA Topoisomerases, Type I,
pubmed-meshheading:6263892-Kinetics,
pubmed-meshheading:6263892-Macromolecular Substances,
pubmed-meshheading:6263892-Molecular Weight,
pubmed-meshheading:6263892-Seeds,
pubmed-meshheading:6263892-Triticum
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pubmed:year |
1981
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pubmed:articleTitle |
Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme).
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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