Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-7-9
pubmed:abstractText
Using partially purified mouse liver 23S mRNA known to be associated with the Ah locus and 3-methylcholanthrene-induced cytochrome P(1)-450, we synthesized double-stranded cDNA by the successive action of reverse transcriptase (RNA-directed DNA nucleotidyltransferase) and the Klenow A fragment of Escherichia coli DNA polymerase I. The double-stranded cDNA was inserted into pBR322 plasmid DNA by Pst I cleavage and homopolymeric "tailing" and cloned in E. coli LE392. Clone 46 hybridized with [(32)P]cDNA made from 23S mRNA from "Ah-responsive" C57BL/6N mice but did not hybridize with similarly prepared [(32)P]cDNA from "Ah-nonresponsive" DBA/2N mice. Clone 30 was positive, and clone 7 was negative, with both C57BL/6N and DBA/2N [(32)P]cDNA probes; these two clones were therefore used as "positive" and "negative" control clones, respectively. By translation-arrest experiments, clone 46 DNA and clone 30 DNA were shown to be associated with anti-P(1)-450- and anti-albumin-precipitable material, respectively. By agarose gel electrophoresis of Pst I digests, the clone 46 DNA insert was shown to be 1100 base pairs in total length and to contain one internal Pst I site. The cDNA made from total mRNA isolated from 3-methylcholanthrene-treated C57BL/6N mice hybridized to the two fragments of Pst I-digested DNA from clone 46, whereas similarly prepared cDNA from 3-methylcholanthrene-treated DBA/2N and control C57BL/6N and DBA/2N mice did not. Of 11 restriction endonucleases used, two (Pst I and Xba I) had sites within the clone 46 DNA insert. After hybridization of clone 46 (32)P-labeled nick-translated DNA to EcoRI fragments from A/HeJ mouse genomic DNA and fractionation by RPC-5 chromatography and gel electrophoresis, only one positive band (3-4 kilobase pairs appeared. These data demonstrate conclusively that pBR322 clone 46 DNA is associated with mRNA controlled by the murine Ah locus, presumably the structural gene encoding 3-methylcholanthrene-induced P(1)-450.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-1055375, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-1105573, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-111241, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-160360, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-221453, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-229404, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-291033, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-344137, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-377492, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-378536, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-383387, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-399809, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-4338637, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-4374548, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-4504350, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-500620, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-55272, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-608592, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-686360, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-6993473, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-6994686, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-6996566, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-762095, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-775445, http://linkedlifedata.com/resource/pubmed/commentcorrection/6262772-823012
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
800-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Isolation and characterization of a cloned DNA sequence associated with the murine Ah locus and a 3-methylcholanthrene-induced form of cytochrome P-450.
pubmed:publicationType
Journal Article