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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1981-7-9
|
| pubmed:abstractText |
Succinyl cyclic adenosine monophosphate (AMP) was synthesized and coupled to beta-D-galactosidase using 1-ethyl-3-(3-dimethylaminopropyl)carbodimide. An antiserum to cyclic AMP was raised in rabbits by immunization with a succinyl cyclic AMP-human serum albumin conjugate. An enzyme immunoassay of cyclic AMP was successfully performed via the competitive binding procedure using the succinyl cyclic AMP-enzyme conjugate and the antiserum to cyclic AMP. The sensitivity of this assay has been increased several hundredfold by prior 2'-O-succinylation of cyclic AMP as already described by Cailla et al. in the radioimmunoassay of cyclic nucleotides. The assay system makes it possible to ascertain values as low as 6 fmol of cyclic AMP/tube. Human plasma cyclic AMP could be accurately determined by this method without requiring a deproteinizing reagent as the first step of assay. The concentration of TCA-extracted cyclic AMP from various tissues of mice was determined by both enzyme immunoassay and radioimmunoassay. There was a good correlation between the values for cyclic AMP determined by the two methods (Y = 1.01X - 0.15, r = 0.996, n = 39). Succinylated nucleotides and nucleosides such as cyclic GMP, ATP, ADP, and AMP, adenosine, adenine, GTP, GDP, GMP, guanosine, and guanine had no effect on the immunoassay of cyclic AMP.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0162-3109
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
53-9
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:6262277-Animals,
pubmed-meshheading:6262277-Antibody Specificity,
pubmed-meshheading:6262277-Cyclic AMP,
pubmed-meshheading:6262277-Galactosidases,
pubmed-meshheading:6262277-Humans,
pubmed-meshheading:6262277-Immunoenzyme Techniques,
pubmed-meshheading:6262277-Rabbits,
pubmed-meshheading:6262277-Radioimmunoassay,
pubmed-meshheading:6262277-beta-Galactosidase
|
| pubmed:year |
1981
|
| pubmed:articleTitle |
Enzyme immunoassay of cyclic adenosine 5', 5' - monophosphate (AMP) using beta-D-galactosidase as label.
|
| pubmed:publicationType |
Journal Article
|