Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-6-23
pubmed:abstractText
A procedure is developed for the isolation of intact chloroplast DNA (ctDNA) from Petunia hybrida. The molecular weight, calculated from contour length measurements, is 96.0 +/- 4.5 x 10(6) daltons. This value is in good agreement with the value of 101.2 x 10(6) daltons that was estimated from the electrophoretic mobilities of restriction endonuclease fragments of ctDNA. Analysis of petunia ctDNA in neutral CsCl gradients revealed the presence of only one type of DNA at a buoyant density of 1.6987 +/- 0.0005 gcm-3. This corresponds with a GC-content of 39.3 +/- 0.5%. A physical map of petunia ctDNA was constructed by using the restriction endonucleases Sal I, Bgl I, Hpa I and Kpn I. The map indicates that petunia ctDNA contains two copies of a sequence in an inverted orientation. The inverted repeat regions have a minimum length of 10 x 10(6) daltons. Hybridization data indicate that part of the inverted repeat regions contain the genes for chloroplast ribosomal RNAs.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-1099983, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-1164522, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-14498379, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-16592373, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-16592612, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-291033, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-403933, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-6244211, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-6253903, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-673835, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-719757, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-745241, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-881736, http://linkedlifedata.com/resource/pubmed/commentcorrection/6261223-890739
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
503-17
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Isolation, characterization and restriction endonuclease mapping of the Petunia hybrida chloroplast DNA.
pubmed:publicationType
Journal Article