|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1981-6-23
|
|
pubmed:abstractText |
EcoRI DNA fragments from a Moloney murine leukemia virus (M-MuLV)-infected mouse fibroblast line (M-MuLV clone A9) were cloned in lambda phage Charon 4A cloning vector to derive clones containing integrated M-MuLV proviral DNA. A 10- to 16-megadalton class of EcoRI fragments was chosen for cloning, based on (i) its ability to induce XC-positive virus upon transfection of NIH/3T3 cells, and (ii) its content of a 0.8-megadalton viral KpnI fragment diagnostic for M-MuLV. Six recombinant DNA clones were isolated which contain a complete M-MuLV provirus, as judged by (i) restriction endonuclease mapping and (ii) the fact that all of the clones gave rise to XC-positive, NB-tropic virus upon DNA infection in NIH/3T3 cells. The sizes of the inserts were 12.0 (for three clones) or 12.5 megadaltons (for three clones). Restriction mapping indicated that these six clones represent five different M-MuLV proviral integrations into different cellular DNA sites.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-1063407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-1251192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-159456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-189309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-203406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-210459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-215325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-221902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-229265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-338419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-4099080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-4368895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-480464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-6153135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-6244569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-6245260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-6248244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-6449003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-660721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-728982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-731793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-7373714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-7432471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-847462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-88264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6260972-948749
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
37
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
181-90
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:6260972-Bacteriophage lambda,
pubmed-meshheading:6260972-Cell Line,
pubmed-meshheading:6260972-Clone Cells,
pubmed-meshheading:6260972-Cloning, Molecular,
pubmed-meshheading:6260972-DNA, Recombinant,
pubmed-meshheading:6260972-DNA, Viral,
pubmed-meshheading:6260972-DNA Restriction Enzymes,
pubmed-meshheading:6260972-Escherichia coli,
pubmed-meshheading:6260972-Fibroblasts,
pubmed-meshheading:6260972-Molecular Weight,
pubmed-meshheading:6260972-Moloney murine leukemia virus,
pubmed-meshheading:6260972-Recombination, Genetic
|
|
pubmed:year |
1981
|
|
pubmed:articleTitle |
Isolation of recombinant DNA clones carrying complete integrated proviruses of Moloney murine leukemia virus.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
