Linked Life Data

6259622

Source:http://linkedlifedata.com/resource/pubmed/id/6259622

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-5-21
pubmed:abstractText
Various base pair specific DNA ligands comprising a phenyl phenazinium dye, a triphenylmethan dye and Hoechst 33258 were covalently bound to polyethylene glycol (PEG) via ester or ether bonds. The DNA interactions of the PEG derivatives formed were shown to exhibit the same base pair specificity as the parent compounds. Since the PEG chains thus bound to the DNA could be expected to increase drastically the frictional coefficient of the DNA, the PEG derivatives were used for base specific DNA separations in agarose and polyacrylamide gel electrophoresis. The procedures, which do not require any special techniques, are described in detail. The resolution observed in agarose gels allows one to separate equally sized DNA fragments differing as little as 1% in base composition at mean travel distances of about 10 cm. Examples of gels showing the base compositional heterogeneity of restriction fragments obtained from lambda DNA, E. coli DNA and calf thymus DNA are given.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-13955687, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-168079, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-215495, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-369706, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-4362628, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-450716, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-5126936, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-593417, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-731693, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-845942, http://linkedlifedata.com/resource/pubmed/commentcorrection/6259622-845955
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
95-119
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Polyethylene glycol derivatives of base and sequence specific DNA ligands: DNA interaction and application for base specific separation of DNA fragments by gel electrophoresis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.