Linked Life Data

6258578

Source:http://linkedlifedata.com/resource/pubmed/id/6258578

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-4-13
pubmed:abstractText
1. (3)H- and (35)S-labelled heparan sulphate was isolated from monolayers of human lung fibroblasts and subjected to degradations by (a) deaminative cleavage and (b) periodate oxidation/alkaline elimination. Fragments were resolved by gel- and ion-exchange-chromatography. 2. Deaminative cleavage of the radioactive glycan afforded mainly disaccharides with a low content of ester-sulphate and free sulphate, indicating that a large part (approx. 80%) of the repeating units consisted of uronosyl-glucosamine-N-sulphate. Blocks of non-sulphated [glucuronosyl-N-acetyl glucosamine] repeats (3-4 consecutive units) accounted for the remainder of the chains. 3. By selective oxidation of glucuronic acid residues associated with N-acetylglucosamine, followed by scission in alkali, the radioactive glycan was degraded into a series of fragments. The glucuronosyl-N-acetylglucosamine-containing block regions yielded a compound N-acetylglucosamine-R, where R is the remnant of an oxidized and degraded glucuronic acid. Periodate-insensitive uronic acid residues were recovered in saccharides of the general structure glucosamine-(uronic acid-glucosamine)(n)-R. 4. Further degradations of these saccharides via deaminative cleavage and re-oxidations with periodate revealed that iduronic acid may be located in sequences such as glucosamine-N-sulphate-->iduronic acid-->N-acetylglucosamine. Occasionally the iduronic acid was sulphated. Blocks of iduronic acid-containing repeats may contain up to five consecutive units. Alternating arrangements of iduronic acid- and glucuronic acid-containing repeats were also observed. 5. (3)H- and (35)S-labelled heparan sulphates from sequential extracts of fibroblasts (medium, EDTA, trypsin digest, dithiothreitol extract, cell-soluble and cell-insoluble material) afforded similar profiles after both periodate oxidation/alkaline elimination and deaminative cleavage.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-1218088, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-123778, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-132967, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-147687, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-176004, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-202253, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-203327, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-4276135, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-6280999, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-6446326, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-820337, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-830558, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-9127, http://linkedlifedata.com/resource/pubmed/commentcorrection/6258578-963010
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
191
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-10
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Structural studies on heparan sulphate from human lung fibroblasts. Characterization of oligosaccharides obtained by selective periodate oxidation of D-glucuronic acid residues followed by scission in alkali.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't