Linked Life Data

6256501

Source:http://linkedlifedata.com/resource/pubmed/id/6256501

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1981-3-24
pubmed:abstractText
Wolfgram protein preparations from myelin are heterogeneous mixtures having major components with molecular weights in the 43,000 to 60,000 range. Through the use of an SDS-slab gel electrophoretic system of high resolving power, the Wolfgram 1 (W1) component described by Nussbaum et al. (1977) has been resolved into two components; bovine W1a and W1b proteins are found to have mobilities identical to those of the two 2',3'-cyclic nucleotide 3'-phosphodiesterase (2'3'-CN 3'-ase) components, CNa and CNb (Drummond, 1979). Crossed immunoelectrophoresis with bovine 2',3'-CN 3'-ase demonstrates that CNa and CNb are antigenically indistinguishable. Rat and bovine Wolfgram proteins W1a and W1b, but not the W2 proteins, display cross reactivity with antiserum directed against bovine 2',3'-CN 3'-ase. Chymotryptic and Staphylococcus aureus V8 protease peptide profiles indicate structural similarities between proteins CNa-W1a and CNb-W1b. It is concluded that an inactivated form of 2',3'-Cn 3'-ase is a major component of Wolfgram protein W1. These results are consistent with the studies on the histochemical localization of rat W1 protein by Roussel et al. (1977, 1978).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1155-65
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Comparison of 2',3'-cyclic nucleotide 3'-phosphodiesterase and the major component of Wolfgram protein W1.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.