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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3-4
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pubmed:dateCreated |
1981-3-27
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pubmed:abstractText |
Commercially available 5-aminosalicylic acid (5-AS) was recrystallised in the presence of Na2S2O5. A completely colourless solution was obtained when the purified product was dissolved at a concentration of 1 mg/ml in a phosphate buffer containing EDTA and H2O2. No significant increase in absorption was found upon storage for 18 h at 4 degrees C. A 8-fold increase in sensitivity of an enzyme-linked immunosorbent assay for the detection of rotavirus antigens was demonstrated by using the modified substrate solution instead of the conventional substrate solution of crude 5-AS. In addition, P/N values did not significantly change between 2 and 18 h after addition of the substrate, thus rendering the time of reading less critical. No difference in sensitivity of the assay was found between modified 5-AS solution and ortho-phenylenediamine (OPD). However, since OPD requires special care in handling the modified 5-AS solution is preferred for use in routine ELISAs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0022-1759
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
325-32
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:6256447-Aminosalicylic Acids,
pubmed-meshheading:6256447-Antigens, Viral,
pubmed-meshheading:6256447-Crystallization,
pubmed-meshheading:6256447-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:6256447-Immunoenzyme Techniques,
pubmed-meshheading:6256447-Mesalamine,
pubmed-meshheading:6256447-Rotavirus
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pubmed:year |
1980
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pubmed:articleTitle |
A simple method for the purification of 5-aminosalicylic acid. Application of the product as substrate in enzyme-linked immunosorbent assay (ELISA).
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pubmed:publicationType |
Journal Article
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