Linked Life Data

6254215

Source:http://linkedlifedata.com/resource/pubmed/id/6254215

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-1-16
pubmed:abstractText
Amoebic antigen prepared from axenic Entamoeba histolytica (NIH:200 strain) grown at 37 degrees C in TPS I medium was chromatographed on a Sephadex G-200 column. Three fractions (F-I, F-II, F-II) were obtained, the molecular weights of which ranged from 650,000 to 1,450. Fractions were concentrated by lyophilization and antisera against each fraction and whole antigen were raised in rabbits. Partially purified fractions were used in various serological tests and their reactivities were compared with whole amoebic antigen. Main haemagglutinating and precipitating activities were confined to Fraction I, which had 14% of total protein and the highest molecular weight (650,000). 5 micrograms/ml of protein contained in this fraction gave antibody titres comparable to those obtained with whole antigen with 40 micrograms/ml or protein. Other fractions, although they had precipitin activity, lacked haemagglutinating activity. The protective efficacies of these fractions are being investigated in our laboratory.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0035-9203
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26-35
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Immunogenicity of axenic Entamoeba histolytica antigen and its fractions.
pubmed:publicationType
Journal Article