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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1980-10-27
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pubmed:abstractText |
The water permeability of V-79 Chinese hamster lung fibroblasts was determined by measuring the rate of cell shrinkage in hypertonic medium using a cell sizer. The water permeability appears to follow Arrhenius kinetics as a function of temperature with a sharp discontinuity at 21 degrees C. An activation energy of 7.0 +/- 1.6 kcal/mole was found below 21 degrees C and 22.8 +/- 3.1 kcal/mole above 21 degrees C. The correlation time of rotation of the spin label 2,2-dimethyl-5-dodecyl-5-methyloxazolidine-N-oxide was measured as a function of temperature in the cellular membranes, and shows a break at 20 degrees C. A discontinuity was also found in the membrane to water partitioning of the spin label 2,2-dimethyl-5-pentyl-5-butyloxazolidine-N-oxide near 20 degrees C. These breaks may correspond to a membrane lipid phase transition. Dimethylsulfoxide, in the concentration range of 0.2--0.5 M, decreases the water permeability by a factor of two.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dimethyl Sulfoxide,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerol,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Sucrose,
http://linkedlifedata.com/resource/pubmed/chemical/Water
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9541
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
103
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
407-16
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:6249829-Animals,
pubmed-meshheading:6249829-Cell Line,
pubmed-meshheading:6249829-Cell Membrane Permeability,
pubmed-meshheading:6249829-Cricetinae,
pubmed-meshheading:6249829-Dimethyl Sulfoxide,
pubmed-meshheading:6249829-Dose-Response Relationship, Drug,
pubmed-meshheading:6249829-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:6249829-Glycerol,
pubmed-meshheading:6249829-Lung,
pubmed-meshheading:6249829-Mathematics,
pubmed-meshheading:6249829-Membrane Lipids,
pubmed-meshheading:6249829-Sucrose,
pubmed-meshheading:6249829-Temperature,
pubmed-meshheading:6249829-Water
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pubmed:year |
1980
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pubmed:articleTitle |
Water permeability of mammalian cells as a function of temperature in the presence of dimethylsulfoxide: correlation with the state of the membrane lipids.
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pubmed:publicationType |
Journal Article
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